Characterization of the putative PP6 of Toxoplasma gondii.

(A) The subcellular localization of TgPP6C and TgPP6R by endogenous tagging shows two subunits localized in the cytoplasm, while TgPP6S was located in the endoplasmic reticulum. Green, TgIMC1 stain denotes the outline of the parasite. Red, mouse anti-HA antibody. Scale bars: 2 μm. (B) IFA shows TgPP6S::6HA (green) colocalized with the endoplasmic reticulum protein TgSERCA (red). Scale bars: 2 μm. (C) Immunoprecipitation (IP) assays were performed to identify TgPP6::6HA subunits (TgPP6C, TgPP6R and TgPP6S) interacting proteins, WT strain as a control. Selected hits identified by LC-MS analyses are shown and the numerical annotations of each gene show the count of unique peptides originating from the MS analysis that matched the corresponding hits. A full list of the hits is provided in S1 Table. (D) TGGT1_203150::6HA parasites were stained with antibodies against TgIMC1 (green) and HA (red). TGGT1_203150 was located in the cytoplasm of the parasite. Scale bars: 2 μm.