Characterization of isolated primary renal cells from NK and CKD kidneys using cell-specific markers.
Florescent antibody staining was carried out on passage 3 (P3) cells. Staining with proximal tubular marker Aquaporin-1 (A-B); Quantitation of proximal tubular cells (Aquaporin-1) among the total isolated primary renal cells at different passages from P3 to P12; (C) Distal tubular marker E-cadherin1 staining of primary renal cells from NK and CKD kidneys. N = 3; (D-E) Quantitation of distal tubular cell (E-cadherin) among the total isolated primary renal cells at different passages from P3 to P12 (F). The overall amounts (percentage) of proximal tubular cells and distal tubular cell were similar in the renal cell population derived from NK and CKD kidneys. Original magnification x20.