pone.0250856.g003.tif (2.83 MB)

Characterization of Pgam1 heterozygous KO mice.

figure

posted on 2021-04-29, 17:29 authored by Takumi Mikawa, Eri Shibata, Midori Shimada, Ken Ito, Tomiko Ito, Hiroaki Kanda, Keiyo Takubo, Atsuyoshi Shimada, Matilde E. Lleonart, Nobuya Inagaki, Masayuki Yokode, Hiroshi Kondoh

(A) The comparison of glycolytic mRNAs profiles between wild-type and Pgam1^+/− mice. The extracts from indicated tissues were tested. (B) Intraperitoneal glucose test (IPGTT) was performed in wild-type (n = 5) and Pgam1^+/− mice (n = 4). (C-E) Several physiological parameters in wild-type (n = 5) and Pgam1^+/− mice (n = 5). (C) Body weight were measured every week for 30 weeks. (D) Measurements of muscle mass and visceral-fat mass by computerized tomography at 35 weeks. (E) Total cholesterol (T-Cho), high-density lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol (LDL-c), Total protein, (TP), albumin (ALB) and blood glucose (Glu) were determined in the blood plasma of 35 weeks mice. +/+; wild-type, +/-; heterozygous knockout. Data represent the mean ± SEM. Single-asterisks (*) and double-asterisk (**) indicate statistical significance of p<0.05 and p<0.005, respectively, Student’s t-test.