pone.0245526.g004.tif (1.75 MB)
Characterization of gene expression between MYCN- and ATRX-clusters.
figure
posted on 2021-01-19, 18:41 authored by Shunsuke Kimura, Masahiro Sekiguchi, Kentaro Watanabe, Mitsuteru Hiwatarai, Masafumi Seki, Kenichi Yoshida, Tomoya Isobe, Yusuke Shiozawa, Hiromichi Suzuki, Noriko Hoshino, Yasuhide Hayashi, Akira Oka, Satoru Miyano, Seishi Ogawa, Junko Takita(A) Differentially expressed genes between MYCN- and ATRX-clusters based on normalized WTS read counts. Green dots represent genes regulated by MYCN. (B) Expression of genes in MES-, ATRX- and MYCN-clusters. Normalized expression was calculated from read counts on each gene with DESeq2 software. The P-value between ATRX- and MYCN-clusters was calculated using the Wilcoxon rank-sum test. The mean and 25th and 75th percentiles are represented in the box plots by the midline and box edges, respectively, and whiskers extend to 1.5 times the interquartile range. Pathway analysis with Metascape software by using top 500 differentially expressed genes in (C) ATRX-cluster and (D) MYCN-cluster. MES, Mesenchymal.
