Characterization of expression pattern of IICs.

A) Dot plot of the expression pattern of IICs in T_regs before and after Nivolumab therapy; B) Venny plot of the significant IICs in T_regs; C) The log₂FC and Adj P.val of IICs in T_regs between two statuses. D) The expression pattern of significant IICs along the pseudo-time; Yellow branches shows highest expression of the gene across the pseudo-time. E) The expression pattern of significant IICs in TCGA tumor bulk dataset, The box plots display the median mRNA expression levels of T_reg markers in HNSCC tumors (represented by the orange plots) and the corresponding normal tissues (represented by the red plot). The values on the axes are represented in units of Log₂ (TPM+1). The * red indicates a p-value less than 0.01, implying statistical significance. The abbreviations T and N denote tumor and normal tissues, respectively.