Characterisation of CENP-C level in control nanos-GAL4, CENP-C RNAi, HA-CENP-C and HA-CENP-C; CENP-C RNAi lines.

Immunofluorescent image of 5-day old (5d) G₂/prophase GSCs (circled) in (A-D) nanos-GAL4 control, (A’-D’) CENP-C RNAi and (A”-D”) HA-CENP-C; CENP-C RNAi stained with DAPI (cyan), CENP-C (green) and 1B1 (red). Scale bar = 5 μm. CENP-C RNAi and rescue experiments were performed at 22°C. (E) Quantitation of total CENP-C fluorescent intensity (integrated density) per GSC in nanos-GAL4, CENP-C RNAi and HA-CENP-C; CENP-C RNAi (rescue). ****p<0.0001, ns = non-significant. Error bars = SEM. (F-I) Immunofluorescence image of 5-day old (5d) G₂/prophase GSCs (circled) over-expressing HA-CENP-C stained with DAPI (cyan), CENP-C (green) and 1B1 (red). * denotes cap cells/GSC niche. GSCs are circled. Scale bar = 5 μm. HA-CENP-A over-expression experiments were performed at 25°C. (J) Quantitation of total CENP-C fluorescent intensity (integrated density) per GSC in HA-CENP-C. ***p<0.001. Error bars = SEM.

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