Changes of autophagic activity in Tz-sensitive (A, C) and -resistant (B, D) breast cancer cells.

Autophagic activity was detected with Western blot analysis and densitometry analysis using LC3B and Beclin-1 antibodies in both cell lines after treatment of Se, Tz, and combinations of both drugs for 72 h. Similar results were observed in replicate experiments. Protein expression levels (relative to β-actin) were determined. One-way ANOVA followed by post Scheffe test was used for statistical analysis to compare between groups. (*P < 0.05; **P < 0.01 significantly different from control; ^# P < 0.05; ^## P < 0.01 significantly different from Tz single treatment). Inhibition of Beclin-1-related autophgic activity was shown in JIMT-1 cell line.