Changes in Her2 and Akt in Tz-sensitive (A, C) and -resistant (B, D) breast cancer cells.

The data are presented as Her2, p-Her2, Akt, and p-Akt expressed relative to control after treatment of Se, Tz, and combinations of both drugs for 72 h by Western blot analysis and densitometry analysis. Similar results were observed in replicate experiments. Protein expression levels (relative to β-actin) were determined. One-way ANOVA followed by post Scheffe test was used for statistical analysis to compare between groups. (*P < 0.05; **P < 0.01 significantly different from control; ^# P < 0.05; ^## P < 0.01 significantly different from Tz single treatment). Effects of Se on Her-2 and Akt in SK-BR-3 and JIMT-1 were opposed to each other.