Changes in EAE-induced cellular infiltration in the optic nerve.

A-D) Representative images of H&E stained sections from control and EAE optic nerves. Increased infiltration was evident in WT EAE sections, while A2 deletion markedly reduced this effect. E-H) High magnification images of boxed regions demonstrate EAE-induced cellular infiltration. Arrows indicate clusters of infiltrated cells. I) Quantification of infiltrated cells using ImageJ. Data are presented as Mean ± SEM. #p<0.01 WT EAE vs WT Con and *p<0.05 A2^-/- EAE vs WT EAE. Number of animals used: 8(WT Control);12 (WT EAE); 9 (A2^-/- EAE); 7 (A2^-/- Control). Representative images are presented. Scale bar 50 μm.