ChIP-seq analysis of ZHX3.

(A) ChIP-seq analysis showing the distribution of ZHX3 around target gene loci in proliferating IMR-90 cells. TSS, transcription start site; TES, transcription end site. (B) Venn diagram showing overlap between ZHX3-enriched genes and genes commonly downregulated in IMR-90 cells undergoing OIS or RS. Transcriptome data of OIS and RS cells were obtained from GSE86546. (C) Gene Ontology analyses of 44 and 51 ZHX3-enriched genes that were upregulated and downregulated, respectively, in both OIS and RS IMR-90 cells. (D) Integrative Genomics Viewer tracks showing the distribution of ZHX3 in a ribosomal DNA complete repeating unit (U13369.1). Data for UBF and RNA Pol IB were obtained from the SRA database under the accession number SRP004897. Bars indicate the PCR amplification sites. ChIP-qPCR analysis of ZHX3 at ribosomal DNA loci in Control- or ZHX3-KD cells (day 3). Values shown are the mean +/- standard deviation from three independent experiments, using the Student’s t-test (*p<0.05, **p<0.01).

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