ppat.1009355.s012.tif (1.89 MB)
CgYps1 is present in the plasma membrane fraction.
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posted on 2021-02-26, 12:51 authored by Anamika Battu, Rajaram Purushotham, Partha Dey, S. Surya Vamshi, Rupinder KaurImmunoblot analysis showing enrichment of CgYps1 and CgPst2 in the plasma membrane fraction of the Q-KO strain expressing CgPST2. Whole-cell lysates and plasma membrane fractions (60 μg and 200 μg for CgPst2 and CgYps1, respectively), prepared by glass bead lysis and sucrose gradient ultracentrifugation, respectively, were resolved on 4–20% polyacrylamide gradient, 12% polyacrylamide, 12% polyacrylamide and 12% polyacrylamide gels for CgYps1, CgPst2, CgPma1 and CgGapdh, respectively, and probed with anti-CgYps1, anti-CgPst2, anti-Pma1 and anti-Gapdh antibodies. The penta-KO strain was used as a negative control.
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arginine -174 residueCgPst 2.flavodoxin-like proteinyeast Candida glabrataimmunoprecipitation-mass spectromet...aspartyl protease-mediated cleavageCgYps 1-CgPst interactionaspartyl protease CgYps 1.implicating CgYps 1CgYps 1 protein levelsNADHstress response mechanismselectron-carrier flavodoxin-fold-co...CgPst 2aids oxidative stress survivalCgYps 1-mediated cleavageglycosylphosphatidylinositol-anchor...CgPst 2 cleavageoxidative stress defense system
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