pgen.1010606.s005.tif (5.61 MB)
Cerebral organoids exhibit neural cortices-like tissue mimic to human brain.
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posted on 2023-02-06, 18:45 authored by Yoshitaka Tamaki, Jay P. Ross, Paria Alipour, Charles-Étienne Castonguay, Boting Li, Helene Catoire, Daniel Rochefort, Makoto Urushitani, Ryosuke Takahashi, Joshua A. Sonnen, Stefano Stifani, Patrick A. Dion, Guy A. Rouleau(A) Representative bright-field (left) and immunofluorescence images (right) of cerebral organoids differentiated from OrgCtrl or OrgALS iPSCs line at 60 DIV. Scale bars = 1 mm. (B) Double-label immunofluorescence images of CTIP2 and PAX6 staining OrgCtrl or OrgALS cerebral organoids at 30 DIV and day 60 DIV. Scale bars = 100 μm. (C) Double-label immunofluorescence images of CTIP2 and TUJ1 (upper panels) or SOX2 (lower panels) staining OrgCtrl or OrgALS cerebral organoids at 60 DIV. Sections were counterstained with DAPI to label the nuclei. Scale bars = 50 μm.
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spinal cord extractsresults provide evidencegenomic damage duepathological feature seenreplicate als pathophysiologyshowed pathogenic tdpderived pathogenic tdphuman cns tissuehuman cerebral organoids43 pathology correlatedals patient inducedcerebral organoidspathogenic tdphuman cnspathological hallmark43 pathologysporadic alsals patientals functionedthree nontemplated propagationtar dnastrand breakspromising strategyprogressively spreadprogressive lossmotor neuronslike propagationform astrogliosisfirst demonstratedfindings indicateeffective therapydna doubledisease progressiondependent mannercytoplasmic mislocalizationcreating readoutsconfirmed experimentallyalso reported
