Cellular fatty acid content after incubation with LA.

Cellular lipids were extracted from 3D hepatic constructs and analyzed as fatty acid methyl esters (FAME). Mean and standard error of the mean (SEM) are reported. Differences between LA (dotted lines) and control (solid line) groups at each time point were analyzed using paired t-tests. N = 12 per group. (A) Hepatocyte LA levels were significantly higher at 4 hours (p = 0.002) and 24 hours (p = 0.03) post-LA diet exposure. (B) DGLA levels did not differ significantly over time or between diet groups (p = 0.27, p = 0.82, respectively). (C) ARA levels were significantly higher at 4 hours after LA diet exposure (p = 0.02). (D) ARA/DGLA ratio was significantly higher in constructs exposed to LA diets at 4 hours (p = 0.008).