ppat.1009430.s002.tif (1.27 MB)

Cell viability at day 5 in the vitro model of monocyte to macrophage differentiation.

figure

posted on 2021-04-06, 17:24 authored by Rajan Guha, Anna Mathioudaki, Safiatou Doumbo, Didier Doumtabe, Jeff Skinner, Gunjan Arora, Shafiuddin Siddiqui, Shanping Li, Kassoum Kayentao, Aissata Ongoiba, Judith Zaugg, Boubacar Traore, Peter D. Crompton

Elutriated monocytes from healthy U.S. adults (n = 9) were incubated for 24 hours with medium alone, uninfected red blood cells (RBC) or Pf-iRBC (monocyte:Pf-iRBC ratio 1:15). At 24 hours, cells were washed and incubated for 3 additional days in human serum plus medium to allow monocytes to differentiate into macrophages (Mf). To quantify cell viability on day five, 10% v/v alamarBlue HS was added to the culture medium of the three populations of macrophages (Mf, RBC-Mf and Pf-iRBC-Mf) for 5 hours and fluorescence intensity (FI) was measured according to the manufacturer’s instructions. FI was normalized to the fluorescence signal in media without cells. Data were analyzed by the Wilcoxon test with Bonferroni adjustment, and levels of significance between the groups are indicated.

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