Cell viability after AdNKX2-1 and AdLacZ infection.

(A) Time-dependent changes after 1000 MOI adenoviral vectors infection in BHP18-21v cells (n = 9). (B) MOI-dependent changes 120 h after adenoviral vector infection (n = 4–9). The ratios of surviving infected cells to non-infected cells at the same timepoint after infection are shown. Error bars indicate SEM. *p<0.05 and **p<0.01, significant differences between AdNKX2-1 and AdLacZ. (C) Western blots showing NKX2-1 expression 72 h after infection with 300 MOI AdLacZ or AdNKX2-1 (upper panel). Loading control, GAPDH expression (bottom panel). L, AdLacZ; N, AdNKX2-1.