Cell cycle state of PAX3 and PAX3-FOXO1 overexpressing embryonic spinal cells.

(A) (i-iii”) GFP and activated CASPASE3 immunodetection and DAPI staining on transverse section of chick embryos 48hpe with the indicated plasmids. (iv) Quantification of the number of activated CASPASE3⁺ cells in the GFP⁺ population in embryos 48hpe with the indicated plasmids (dots: embryo values; bar plots: mean ± s.e.m.). (B) GFP and MCM2 immunodetection and DAPI staining on transverse sections of chick embryos 48hpe with the indicated plasmids. (iv) Quantification of MCM2 levels in the GFP⁺ cells 48hpe with the indicated plasmids (dots: single cell values; bar plots: mean ± s.e.m.; n>5 embryos). (C) Heatmaps indicated fold changes in the expression of the indicated genes relative to their mean expression in pCIG samples assayed in FAC sorted GFP⁺ from chick embryos 48hpe with the indicated constructs. (D) Quantification of the number of PH3⁺ cells in the GFP⁺ population in embryos 24hpe with the indicated plasmids (dots: embryo values; bar plots: mean ± s.e.m.). x’ and x” panels are blown up on a subset of x panel GFP⁺ cells. Mann-Whitney U test p-value: *: p< 0.05, **: p<0.01, ***: p<0.001, ****: p<0.0001, ns: p>0.05. Scale bars: 50μm.

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