Cassette stably integrated into the genome of the HEK dCas9-SAM_Renin-luciferase cells.

The cassette contains the Firefly luciferase, which was used as a reporter for REN expression. Puromycin was used to select the cells. The homology arms were required for the correct in frame insertion of the cassette. The elements G418-resistance and thymidine-kinase also contained in the cassette were not used in this study.

(TIF)