Cardiac-specific p38γ/δ^act overexpression decreases glycolysis but does not alter expression of enzymes involved in lipid metabolism or mitochondrial function.

Mice were IV injected at PD1 with AAV-cTnT-GFP-Luc (TnTGFP) or AAV-cTnT-p38γ/δ_act (TnTp38/δ^act) and killed at PD14. (A) Immunoblot expression analysis of lipid metabolic enzymes in cardiac tissue with its respective quantification. (B) Cardiac mitochondrial to chromosomal DNA ratio. (C) Immunoblot of mitochondrial complexes in heart lysates. (D) Cardiac mitochondrial ATP production, measured upon exposure to glutamate plus malate or free fatty acids. (E) qRT-PCR of enzymes involved in cardiac lipid metabolism. Data are mean ± SEM (n = 10–15). *p < 0.05, ***p < 0.001 by Student t test. Raw data are given in S14 Fig. qRT-PCR, real-time quantitative PCR.

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