CG9129 and crq regulate each other at the transcriptional level during the clearance of ACs in S2 cells.

A. The CG9129 mRNA levels were quantified by qPCR in Crq-overexpressing S2 cells (Crq-GFP) and control S2 cells. The crq mRNA levels were also quantified by qPCR in CG9129 over-expressing S2 cells (CG9129-flag) and control S2 cells. 0 h refers to S2 cells in the absence of ACs; 6 h refers to S2 cells co-incubated with ACs for 6 h. RpL32 was used as the internal control. B. The CG9129 mRNA levels were also determined in RNAi-treated S2 cells (control and for the knockdown of crq) after co-incubation with ACs for 6 h, by qPCR. Statistical significance was assessed using the two-way ANOVA (A) or Student’s t-test (B).

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