ppat.1007328.g003.tif (1.03 MB)

BriC does not influence early biofilm development.

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posted on 2018-10-11, 18:39 authored by Surya D. Aggarwal, Rory Eutsey, Jacob West-Roberts, Arnau Domenech, Wenjie Xu, Iman Tajer Abdullah, Aaron P. Mitchell, Jan-Willem Veening, Hasan Yesilkaya, N. Luisa Hiller

(A) Representative confocal microscopy images showing top view of the reconstructed biofilm stacks of WT, ΔbriC and ΔbriC::briC cells of strain R6D stained with SYTO59 dye at 24h. Images are pseudo-colored according to depth (scales shown). (B) COMSTAT2 quantification of 24h biofilm images. Y-axis denotes units of measurement: μm3/μm2 for biomass, and μm for maximum thickness and average thickness over biomass. Error bars represent standard error of the mean calculated for biological replicates (n = 3); “ns” denotes non-significant comparisons, **** p<0.0001 using ANOVA followed by Tukey’s post-test.