BriC does not influence early biofilm development.
(A) Representative confocal microscopy images showing top view of the reconstructed biofilm stacks of WT, ΔbriC and ΔbriC::briC cells of strain R6D stained with SYTO59 dye at 24h. Images are pseudo-colored according to depth (scales shown). (B) COMSTAT2 quantification of 24h biofilm images. Y-axis denotes units of measurement: μm3/μm2 for biomass, and μm for maximum thickness and average thickness over biomass. Error bars represent standard error of the mean calculated for biological replicates (n = 3); “ns” denotes non-significant comparisons, **** p<0.0001 using ANOVA followed by Tukey’s post-test.