Blocking assay for VV entry by soluble B2M.

V-e.Luc virus was incubated with increasing concentrations of soluble BSA (control) or B2M (0, 5, 15, 30 and 50 μg/mL) protein for 1 h at room temperature. Then, HeLa cells were incubated for 1 h with the pre-treated inoculum. At 3 h.p.i. luciferase activity was determined as a measure of viral entry and early gene expression. No significant differences were found. p-values: **** < 0.0001, *** < 0.001, ** < 0.01, * < 0.05, ns > 0.05.

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