ppat.1009314.s009.tif (916.98 kB)
Biolayer interferometry (BLI) tracings for binding of FimHLD to immobilized DSG2 EC1-5 expressed from WT Expi293F cells (left panel) or Expi293F GnTI- cells (right panel).
figure
posted on 2021-01-29, 18:42 authored by Lisa K. McLellan, Michael R. McAllaster, Arthur S. Kim, Ľubomíra Tóthová, Patrick D. Olson, Jerome S. Pinkner, Allyssa L. Daugherty, Teri N. Hreha, James W. Janetka, Daved H. Fremont, Scott J. Hultgren, Herbert W. Virgin, David A. HunstadA 1:1 binding model (red lines) was used to fit experimental curves (black lines). Representative curves shown from 5 independent BLI runs per condition; affinity values represent the mean ± SD of two independent experiments.
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