Behavioral consequences of PDFR expression in l-LNvs.

(A) Daytime sleep in 5-day-old l-LNv-GAL4>; UAS-Pdfr^WT/+ flies and parental controls (ANOVA_[2,92] = 5.7 p = 0.004; n = 30–32/genotype). (B) Sleep in Dcr2; c929-GAL4/UAS-Pdfr flies and parental controls (ANOVA_[2,150] = 17.24 p = 1.86^E-07; n = 40–60/genotype; *p < 0.05, modified Bonferroni test). (C) Kaplan–Meier analysis reveals % survival during starvation in l-LNv-GAL4>; UAS-Pdfr^WT flies and parental controls (n = 3 replicates of 10–16/genotype, χ² = 19.55, df = 2, p-value < 0.0001). (D) Kaplan–Meier analysis reveals % survival during starvation in Dcr2; c929-GAL4/UAS-Pdfr^RNAi flies and parental controls (n = 3 replicates of 10–16/genotype, χ² = 23.35, df = 2, p-value < 0.00001). Data underlying this figure can be found in S4 Data. l-LNv, large ventral lateral neuron; n.s., not significant; PDFR, pigment dispersing factor receptor.