BR inhibits the synthesis of superoxide anions through the peroxidase pathway.
The length of the primary root of WT and det2-9 seedlings when exposed to inhibitors of NADPH oxidase (A) diphenylene iodonium, (B) ZnCl2. Asterisks means significant difference from the control-treated plants (**P<0.01). (C) NBT staining of WT and det2-9 when exposed to salicylhydroxamic acid (SHAM), 1,10-phenanthroline (1,10-Phe), diphenylene iodonium (DPI) and ZnCl2. Bar = 50 μm. (D) Peroxidase activity in WT and det2-9 nine-day old seedlings treated or not with eBL (10 nM). Data shown are mean±SE (n = 6); **: means differ significantly (P<0.01).