BRG1 knockdown impairs colony formation and modulates cyclin family.

(A,B) Colony formation assay was performed to analyse proliferation of both human HCC cell lines HepG2 and HuH7. Colony formation was reduced significantly in both cell lines, HepG2 and HuH7, n = 3. (C) Analysis of mRNA expression by qRT-PCR showed a significant down-regulation of cyclinB and cyclinE expression in BRG1-suppressed HepG2 cells at the time point of 40h after transfection, n = 4. (D) HuH7 cells showed a trend towards lower expression though this was not significant, n = 4. Negative control was standardised as 1.