pone.0246647.s005.tif (1.37 MB)
BEARmix amplification curves for different input RNA amounts (related to Fig 4A).
figure
posted on 2021-02-03, 18:59 authored by Thomas G. W. Graham, Claire Dugast-Darzacq, Gina M. Dailey, Xammy H. Nguyenla, Erik Van Dis, Meagan N. Esbin, Abrar Abidi, Sarah A. Stanley, Xavier Darzacq, Robert TjianCurves for individual wells are shown in blue (upper row; non-hot-start BEARmix) or red (lower row; hot-start BEARmix). Means are shown in black. Each column corresponds to a different (average) number of RNA molecules per reaction. Linear background subtraction was performed, using the first 15 cycles to establish the baseline drift.
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