Attachment and invasion of T6SS mutants and EiWT to CCO.

The mean photon emission was obtained from each mutant and positive (EiWT) and negative (E. coli DH5α and cell only) controls. The data represent the mean ±SD from one experiment. The experiment included three replicate wells for each mutant and control. Negative control was used to subtract background noise bioluminescence from each sample. (A) A representative image of bioluminescent imaging of CCO cells treated with bioluminescent T6SS mutants. (B) The mean photon emission shows the attachment ability of T6SS mutants and EiWT. The lowercase letters show significant differences between treatments (p < 0.05). (C) The mean photon exposure was obtained from the same 24-well plate, including gentamycin, for an hour after attachment.