posted on 2021-09-16, 17:36authored byUchenna Watson Waturuocha, Athira P. J., Krishna Kumar Singh, Vandana Malhotra, M. S. Krishna, Deepak Kumar Saini
A-G. Relative expression of mRNA levels in H37Ra strains over-expressing the wild-type or mutant mtrB cloned in the pMV261 vector and grown in Middlebrook 7H9 medium. The expression of the specific genes was normalized to the levels of 16S rRNA before calculating the fold change between test and vector control samples. The expression changes are shown for the A.mtrB; B.mtrA; C.ilvE; D.ftsQ; E.murG; F.ftsW; G.ftsZ. For all experiments, n = 3 biologically independent experiments. (p values; *≤ 0.05, **≤ 0.01, ***≤ 0.001, ****≤ 0.0001).