pone.0247134.g002.tif (881.14 kB)
Assembly of His-pVP2 into supramolecular structures in plant tissues.
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posted on 2021-02-16, 18:38 authored by Carla Marusic, Charifa Drissi Touzani, Alessio Bortolami, Marcello Donini, Claudia Zanardello, Chiara Lico, Emile Rage, Siham Fellahi, Mohammed El Houadfi, Calogero Terregino, Selene BaschieriA) ELISA with an anti-VP2 serum of the sucrose cushion resuspended pellets. His-pVP2: pellet obtained with the His-pVP2 extract; C-: pellet obtained with the p19 extract; C+: inactivated IBDV. The reported values are the mean of three independent experiments, and error bars represent SD of the means. B) TEM images of the plant-produced VP2-based particles. In the upper panel, the different spherical particles and their measured diameters are indicated. The example of a tubular structure is shown in the lower panel.
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novel subunit vaccinesInfectious Bursal Disease Virusform supramolecular structuresBursal Disease Virusspecific-pathogen-free chicksIBDV strainIBDV neutralizing antibodiesintramuscular immunizationantibodyvirus-like particlesGumboro diseaseVP 2Nicotiana benthamianainsect cellscauses mortalityhistomorphological alterationscapsid protein VP 2transmission electron microscopyVP 2 proteinsucrose density gradient fractionsetiological agentVP 2-based particles400 nmpoultry industry
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