Apoptosis and necrosis detection in BHP18-21v cells after adenoviral vector infection.

(A) Acridine orange- and ethidium bromide-stained cells. The green, orange, and magenta arrows represent early apoptotic (acridine orange-stained condensed chromatin), late apoptotic (ethidium bromide-stained condensed chromatin), and necrotic (ethidium bromide-stained normal chromatin) cells, respectively. (B) Hoechst 33342-stained cells. The green and magenta arrows represent an apoptotic cell with a condensed nucleus and a necrotic cell with a ballooning nucleus, respectively. (C) Real-time assay using annexin V (n = 3). The luminescence and fluorescence signals indicate apoptosis and necrosis, respectively. Error bars indicate SEM. *p<0.05 and **p<0.01, significant differences between AdNKX2-1 and AdLacZ. (D) Caspase 3/7 activity-dependent fluorescence intensity 72 h after infection (n = 6). Error bars indicate SEM. **p<0.01, significant differences between AdNKX2-1 and AdLacZ.