ppat.1012079.s007.tif (1.3 MB)
Analysis of the effects of TLR2 and TLR4 silencing on macrophages.
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posted on 2024-03-11, 17:41 authored by Yumei Li, Christine Wu, Jiyoung Lee, Qiqi Ning, Juhyeon Lim, Hyungjin Eoh, Sean Wang, Benjamin P. Hurrell, Omid Akbari, Jing-hsiung James Ou(A) THP-1 macrophages with shNS, shTLR2 or shTLR4 silencing were analyzed for the RNA level of TLR2 and TLR4 by RT-qPCR. (B) Immunoblot analysis of THP-1 macrophages with shNS, ShTLR2 or shTLR4 silencing. (C) Kupffer cells treated with control siRNA (siNS), TLR2 siRNA (siTLR2) or TLR4 siRNA (siTLR4) were lysed for analysis of mouse TLR2 (mTLR2) and TLR4 (mTLR4) RNAs by RT-qPCR. (D) Human MDMs treated with siNS, siTLR2 or siTLR4 were lysed for RNA analysis by RT-qPCR. (E) Quantitative analysis of the metabolic parameters of the OCR study shown in Fig 7H. In (A) and (C-E), N.S., not significant; *, p<0.05; **, p<0.01; ***, p<0.001.
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tricarboxylic acid cycleopposite metabolic activitieslow oxidative phosphorylationlow glycolytic activitiesimportant metabolic intermediatehigh glycolytic activitieshepatitis b virusatypical metabolism inducedassociated protein 3xlink "> macrophagesreprogram mitochondrial metabolismlike receptor 4hbv e antigenhbeag also enhancesdisplay high oxphoslike macrophages inducedlike proinflammatory polarizationmacrophages like macrophagesmacrophages dependingmitochondrial genesinflammatory polarizationlike antivia tollpromote oxphosinduce deathhbv attenuateshbeag leadshbeag ),antiviral response
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