pone.0260878.s003.tif (1014.6 kB)
Analysis of slit membrane proteins of PodoPalld129 mouse glomeruli.
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posted on 2021-12-08, 18:29 authored by Nadine Artelt, Alina M. Ritter, Linda Leitermann, Felix Kliewe, Rabea Schlüter, Stefan Simm, Jens van den Brandt, Karlhans Endlich, Nicole Endlich(A) Quantitative analysis of nephrin mRNA levels in isolated glomeruli showed no significant difference between PodoPalld129-/- mice and controls (mean±SD; unpaired Student’s t-test). (B) Immunofluorescence staining of kidney sections reveal a slightly decreased expression of the slit membrane protein podocin in PodoPalld129-/- mice compared with corresponding controls. Scale bar represents 20 μm. (C) However, we found no significant difference of podocin mRNA in isolated glomeruli of PodoPalld129-/- and PodoPalld129+/+ mice (mean±SD; 6 months: Mann-Whitney U test, 12 months: unpaired Student’s t-test).
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parietal epithelial cells129 genetic backgroundmorphological deviations like12 months showedchronic kidney diseasesbinding protein palladin>, three palladinpodocyte foot processesdiv >< pbinding partners pdlim26 genetic backgroundproper actin cytoskeletonsignificantly reduced expressionproper podocyte morphologypodopalld129 -/- micepodopalld129 -/-podocyte morphologyreduced expressionmorphological changesimportant actinpodopalld129 -/-)kidney dependskidney damageintact morphologypodocyte spacetaken togetherspecific knockoutresults showproteinuria followedpdlim2 marked dilatationinteracting proteinsglomerular tuftfiltration barrierenlarged subclose contactback crossedage showed6 -/-)
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