pone.0260878.g004.tif (3.06 MB)
Analysis of palladin interacting proteins in PodoPalld129 mice.
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posted on 2021-12-08, 18:29 authored by Nadine Artelt, Alina M. Ritter, Linda Leitermann, Felix Kliewe, Rabea Schlüter, Stefan Simm, Jens van den Brandt, Karlhans Endlich, Nicole Endlich(A) Immunofluorescence stainings of kidney sections show a reduced expression of Pdlim2 and pLasp-1 in PodoPalld129-/- mice compared with corresponding controls. No difference was found for ezrin. Scale bar represents 20 μm. (B) A significant downregulation of Pdlim2 mRNA in 6 months old PodoPalld129-/- glomeruli was found by qRT-PCR. Lasp-1 and Ezrin were not significantly changed. (C) Relative protein level in 6 months old PodoPalld129-/- glomeruli normalized to Gapdh and protein lysates of PodoPalld129+/+ glomeruli. Data are presented as means±SD; *** p<0.001; ns, not significant; unpaired Student’s t-test.
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parietal epithelial cells129 genetic backgroundmorphological deviations like12 months showedchronic kidney diseasesbinding protein palladin>, three palladinpodocyte foot processesdiv >< pbinding partners pdlim26 genetic backgroundproper actin cytoskeletonsignificantly reduced expressionproper podocyte morphologypodopalld129 -/- micepodopalld129 -/-podocyte morphologyreduced expressionmorphological changesimportant actinpodopalld129 -/-)kidney dependskidney damageintact morphologypodocyte spacetaken togetherspecific knockoutresults showproteinuria followedpdlim2 marked dilatationinteracting proteinsglomerular tuftfiltration barrierenlarged subclose contactback crossedage showed6 -/-)
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