Analysis of “blind” or “left out” sets of subject sera samples using training a set composed of CABG patients versus control individuals.

(A) Training set of serum discrimination of CABG patients (triangles) from controls (circles) by % of CABG LOOCV classified mass peaks; one false negative is observed. (B) Non-serum sample discrimination observed when the two different sample groups in panel A are mixed together randomly followed by the same LOOCV mass peak analysis. (C) Assessing the ability of the training set in panel A to correctly discriminate a blinded group of ten samples; 9 out of 10 samples were correctly identified. (D) Receiver operator characteristic (ROC) curve analysis of LOOCV data in Fig 4 and Fig 5. ROC analysis exhibits high sensitivity (true positive rate) of the group binary comparisons in Fig 4 panel A and Fig 5 panel A, and the near random results for the inter-group sample randomizations in Fig 4 panel B and Fig 5 panel B; the horizontal line exhibits a complete random result.