Analysis of ZHX3-knockdown cells.

(A) RT-qPCR analysis of ZHX3 expression on day 3 in IMR-90 cells undergoing ZHX3-KD compared with Control-KD. (B) Western blot analysis of ZHX3 on day 1 and day 2 of Control- or ZHX3-KD in IMR-90 cells. (C) Proportions of IMR-90 cells containing one or two nucleoli in Control- and ZHX3-KD. The number of nucleoli/cell was calculated by measuring the number of fluorescence signals of nucleophosmin (B23) in a single cell (each n >1,000 cells). (D) RT-qPCR analysis of p14/ARF on day 3 of ZHX3-KD compared with Control-KD in IMR-90 cells. (E) RT-qPCR analysis of p16^INK4a on day 3 of ZHX1-, ZHX 2- or ZHX 3-KD compared with Control-KD in IMR-90 cells. Values shown are the mean +/- standard deviation from three independent experiments, using the Student’s t-test (*p<0.05, **p<0.01).

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