Analysis of M1-like and M2-like markers of THP-1 macrophages and human MDMs and quantitative analysis of the metabolic parameters of OCR and ECAR.

(A) THP-1 macrophages (THP-1(M0)) were treated with LPS and IFN-γ (THP-1(LPS+IFN-γ), with IL-4 and IL-13 (THP-1(IL-4+13)), with the incubation medium of Huh7 cells that had been infected with HCV (THP-1(M0)+HCV) for 24 hours, or with the incubation medium of Huh7 cells that had been transfected with the 1.3mer HBV genomic DNA (THP-1(M0)+HBV) for 48 hours. Cells were then lysed for the analysis of IL-1β, TNF-α, iNOS, CD163, IL-10 and MRC-1 RNAs by RT-qPCR. (B) Human MDMs were treated with GM-CSF (M(GM-CSF) or CSF-1 (M(CSF-1) for the induction of M1-like or M2-like polarization, respectively. In addition, M(GM-CSF) were also treated with HCV or HBV. Cells were then lysed for analysis of IL-1β, TNF-α, iNOS, CD163, IL-10 and MRC-1 RNAs by RT-qPCR. (C-D) and (E-F) are the quantitative analyses of the metabolic parameters of OCRs and ECARs of Fig 1A and 1B, respectively. N.S., not significant; *, p<0.05; **, p<0.01; ***, p<0.001.

(TIF)