ppat.1010928.s009.tif (603.2 kB)

Analysis of LOS production in altered phage susceptibility mutants bearing mutations in capsule-control loci or lon.

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posted on 2023-06-08, 17:46 authored by Jinna Bai, Nicole Raustad, Jason Denoncourt, Tim van Opijnen, Edward Geisinger

LOS production in cell lysates of mutants in bfmRS (A), rnaA and dsbA (B), and lon (C) was analyzed in comparison with WT control via Pro-Q 300 emerald staining. Representative gels are shown in top panels. Arrowhead indicates location of novel intermediate band associated with ∆bfmS (A). Quantification is shown in bottom panels. Bars show mean ± s.d. (n = 3). Within each set of strains, no significant difference (P > 0.05) observed in total LOS levels by one-way ANOVA (A, B) or unpaired t-test (C).

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t2 rnase protein spontaneous resistance mapping resistant nosocomial pathogen information could lead improved antimicrobial therapies gained renewed interest div >< p global regulator bfmrs phage remains limited determine phage infectivity identified novel bfmrs blocking loki infection capsule modulation provides lytic phage loki alter capsule structure acinetobacter baumannii loki infection baumannii acinetobacter capsule modulation structural modulation phage challenge reducing capsule loki uses loki replication loki adsorption k3 capsule capsule synthesis phage susceptibility work demonstrates wide determinants using tn unknown mechanisms transcriptional regulation threatening infections studies focused results support opposite effect major determinant key center including knockouts inactivating mutations identified genome hypersensitize bacteria host killing essential receptor adjunctive treatment activating mutations

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