ppat.1010928.s009.tif (603.2 kB)

Analysis of LOS production in altered phage susceptibility mutants bearing mutations in capsule-control loci or lon.

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posted on 2023-06-08, 17:46 authored by Jinna Bai, Nicole Raustad, Jason Denoncourt, Tim van Opijnen, Edward Geisinger

LOS production in cell lysates of mutants in bfmRS (A), rnaA and dsbA (B), and lon (C) was analyzed in comparison with WT control via Pro-Q 300 emerald staining. Representative gels are shown in top panels. Arrowhead indicates location of novel intermediate band associated with ∆bfmS (A). Quantification is shown in bottom panels. Bars show mean ± s.d. (n = 3). Within each set of strains, no significant difference (P > 0.05) observed in total LOS levels by one-way ANOVA (A, B) or unpaired t-test (C).