pone.0293845.s004.tif (1.18 MB)
Amplification of endogenously infected blood samples by IOME RT-QuIC.
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posted on 2023-11-02, 17:55 authored by Charlotte M. Thomas, M. Khalid F. Salamat, Christopher de Wolf, Sandra McCutcheon, A. Richard Alejo Blanco, Jean C. Manson, Nora Hunter, E. Fiona Houston(A) Whole blood (WB) and (B) buffy coat (BC) samples (5–10 μl) from BSE-infected (+ve) sheep (animal ID: N257 at clinical time point) and mock-infected (uninfected) sheep (animal ID: N214 at 0 mpi) were tested by a modified IOME RT-QuIC assay but failed to produce positive results. The mean fluorescence from n = 4 replicate reactions is plotted over a period of 80 h.
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