The amino acid sequence alignment was generated using ClustalW. Identical amino acids and similar amino acids are marked with a black and a grey box, respectively. The conserved cysteine residues are labelled with red arrows. The blue bar over the alignment indicates the N-terminal domain (blue), the linker region (yellow) and the C-terminal domain (green) based on the structure of FdhD (PDB: 4pde) [19].