posted on 2011-12-31, 14:33authored byNiek P van Til, Kirstin M Heutinck, Roos van der Rijt, Coen C Paulusma, Michel van Wijland, David M Markusic, Ronald PJ Oude Elferink, Jurgen Seppen
O western blotting as described. Lanes represent: (1) wild type ABCB4, (2) mutant ABCB4, (3) negative control and (4) ABCC1. Wild type and mutant ABCB4 migrated at an apparent molecular mass of 140 kDa. ABCC1 migrated at a molecular mass of 170 kD. The two bands in both ABCB4 and ABCC1 lanes most likely correspond to differently glycosylated forms. Wild type and mutant ABCB4 appear to be differently processed. Similar amounts of cellular protein were loaded as shown by β-actin antibody reactivity.
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Taken from "Alteration of viral lipid composition by expression of the phospholipid floppase ABCB4 reduces HIV vector infectivity"