posted on 2011-12-31, 14:33authored byNiek P van Til, Kirstin M Heutinck, Roos van der Rijt, Coen C Paulusma, Michel van Wijland, David M Markusic, Ronald PJ Oude Elferink, Jurgen Seppen
N equal amounts of HIV p24 were loaded on the gel, the intensity of VSVg staining is also identical. With loading of equal amounts of transducing units (TU) the intensity of VSVg staining is much stronger in the virus produced with ABCB4. This indicates that ABCB4 expression during production of viral vectors does not compromise VSVg incorporation.
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Taken from "Alteration of viral lipid composition by expression of the phospholipid floppase ABCB4 reduces HIV vector infectivity"