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Akt inhibitors as an HIV-1 infected macrophage-specific anti-viral therapy-3

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posted on 31.12.2011, 14:33 authored by Pauline Chugh, Birgit Bradel-Tretheway, Carlos MR Monteiro-Filho, Vicente Planelles, Sanjay B Maggirwar, Stephen Dewhurst, Baek Kim
Rease cell survival by preventing PTEN from binding to p53. Binding of HIV-1 Tat to p53 may result in reduced levels of both p53 by destabilization and PTEN by downregulation of PTEN expression. In vitro binding assay: Lysates containing p53-FLAG were incubated with BSA (control) or HIV-1 Tat protein and then mixed with lysate containing PTEN V5-tag. Proteins bound to p53 were immunoprecipitated using anti-FLAG immobilized antibody and analyzed for PTEN-V5 tag levels by Western blotting. Ratios of PTEN-V5 levels normalized by p53-FLAG levels are shown.

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Taken from "Akt inhibitors as an HIV-1 infected macrophage-specific anti-viral therapy"


Retrovirology 2008;5():11-11.

Published online 31 Jan 2008



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