posted on 2011-12-31, 14:33authored byPauline Chugh, Birgit Bradel-Tretheway, Carlos MR Monteiro-Filho, Vicente Planelles, Sanjay B Maggirwar, Stephen Dewhurst, Baek Kim
Roteins and EGFP (+) or heat-inactivated vector (-). Levels of PTEN protein in YU-2 infected macrophage and HIV vector-transduced macrophages as determined by Western blotting. Ratios of PTEN normalized by β-tubulin levels are shown. Reverse transcriptase PCR analysis of PTEN mRNA levels following transduction of macrophage with HIV vector (+) or treatment with heat-inactivated vector (-). M: 100 bp size marker. β-tubulin and β-Actin were used for loading controls in the Western analysis and RT-PCR, respectively.
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Taken from "Akt inhibitors as an HIV-1 infected macrophage-specific anti-viral therapy"