ppat.1009165.s007.tif (7.65 MB)

Affinity measurements of anti-SARS-CoV-2 mAbs.

figure

posted on 2021-02-11, 18:28 authored by Michael Mor, Michal Werbner, Joel Alter, Modi Safra, Elad Chomsky, Jamie C. Lee, Smadar Hada-Neeman, Ksenia Polonsky, Cameron J. Nowell, Alex E. Clark, Anna Roitburd-Berman, Noam Ben-Shalom, Michal Navon, Dor Rafael, Hila Sharim, Evgeny Kiner, Eric R. Griffis, Jonathan M. Gershoni, Oren Kobiler, Sandra Lawrynowicz Leibel, Oren Zimhony, Aaron F. Carlin, Gur Yaari, Moshe Dessau, Meital Gal-Tanamy, David Hagin, Ben A. Croker, Natalia T. Freund

(A) SPR sensograms showing binding of injected SARS-CoV-2 RBD at six different concentrations (15.6 nM, 31.25 nM, 62.5 nM, 125 nM, 250 nM and 500 nM) to immobilized anti-SARS-CoV-2 TAU mAbs (0.5 μg/ml). mGO53 was used as isotype control. SPR assays were performed on a Biacore T200 instrument at 25°C. Three replicates were performed for each mAb and all samples were diluted in HBS-EP buffer (0.01 M HEPES, 0.15 M NaCl, 0.003 M EDTA, 0.05% Tween 20, pH 7.4). Sensograms were fitted to 1:1 binding model using non-linear regression in the biaevaluation software. K_D was calculated using the ratio of the kinetic constants K_D = K_d/K_a. (B) Rmax, KD, and U-value values.

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