Adoptive transfer of BRD509-immune splenocytes fails to protect cell recipients from lethal challenge.

B6 mice (6–8 weeks old) were infected i.v. with 200CFU BRD509 strain. (A) Bacteria counts on solid LB media from homogenate spleen of infected mice over the course of the primary infection. (B) Survival of mice immunised with BRD509 i.v., or uninfected, and 14 weeks later challenged with 200CFU SL1344 i.v.. (C) Survival of groups of mice that adoptively received 5×10⁷ total donor splenocytes harvested at weeks 1, 2, 3, 4, 6, 14 post-single BRD509 infection and 24h post-transfer were challenged with 200CFU SL1344 iv. (D) SL1344 counts on differential, solid XLD media from spleen and liver of B6 mice recipients of week 2 or naïve donor splenocytes on day 4 post-challenge. (E) Survival of either complete (B6) of lymphocyte-deficient (Rag2^-/- Il2rg^-/-) mice that adoptively received 5×10⁷ total naïve or week 2 BRD509 donor splenocytes and 24h post-transfer were challenged with 200CFU SL1344 i.v.. Data is representative of (A,B,E) 2–4 and (C,D) 2 pooled independent experiments with (A) 4–8, (B) 10, (C) 5–7, (D) 5–10 and (E) 6–11 samples per group. (A) Symbols and bars represent mean and SEM, (B,C,E) symbols represent percentage of survivors, and (D) lines and symbols represent mean and individual measurements. respectively. Statistical analysis, log-rank (Mantel-Cox), multiple t-student test. ***p<0.005, ****p<0.001.

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