Additional file 6 of Peptide targeting of lysophosphatidylinositol-sensing GPR55 for osteoclastogenesis tuning

Additional file 5. Figure S3. Effects of GPR55 modulators on osteoclast maturation. (a, b) Real-time PCR analysis of the differentiation markers (as indicated) in RAW264.7 cells treated with 15 ng/mL RANKL for 72 h, in the absence or presence of 1 µM soybean LPI alone or with GPR55 antagonists (0.5 µM ML-191; 0.5 µM CID16020046). (c) Real-time PCR analysis of Ctr in RAW264.7 cells interfered with non-targeting (si-NT) or Gpr55-targeting (si-GPR55) siRNAs, and subsequently treated with 15 ng/mL RANKL for 72 h in the absence or presence of 1 µM soybean LPI or 1 µM ML-184. The transcripts were quantified and normalised using β2-microglobulin expression, as the housekeeping gene. Data are expressed as proportions (%) of the corresponding control RANKL, as means ±SEM from at least three independent experiments. *p <0.05, **p <0.01 (Student’s t-tests). RANKL, RANKL-differentiated cells. CID, CID16020046.