Additional file 6 of A nuclear function for an oncogenic microRNA as a modulator of snRNA and splicing

Additional file 6 Supplementary Fig. 6. miR-10b regulates U6 snRNA but not U2 and U4 snRNAs in glioma cells. a Representative denaturing Northern blotting of glioma cells and GSCs transfected with either U6 ASOs, miR-10b inhibitor or mimic, with probes specific for U2 and U4 snRNAs. b miR-10b mimic and inhibitor do not regulate 3′ UTR luciferase reporters bearing full-length U6 snRNA sequence in glioma cells. The data is presented as Renilla/Firefly relative luminescence and normalized to the corresponding values in cells not transfected with the oligonucleotides. n = 12; Graphical data are shown as mean ± SEM. c qRT-PCR analysis of snRNA U6 levels in U251 cells transfected with either miR-10b inhibitor, mimic, or corresponding controls, and treated with 5 μg/ml Actinomycin D (mean ± SD, n = 3). P values were calculated using two-way ANOVA. d U251 cells were treated with Actinomycin D followed by the qRT-PCR analysis of miR-10b and U6 levels (mean ± SD, n = 3). P values were calculated using two-way ANOVA. e LN229 and U251 cells were treated with Actinomycin D followed by WST-1 analysis at the indicated time points (mean ± SD, n = 3). P values were calculated using two-tail unpaired t-test. * P < 0.05; **** P < 0.0001.