Additional file 5 of STOML2 potentiates metastasis of hepatocellular carcinoma by promoting PINK1-mediated mitophagy and regulates sensitivity to lenvatinib

Additional file 5: Figure S3. STOML2 promotes mitophagy in HCC cells under stress. (A–B) Confocal microscopy was performed to detect spatial colocalization of mitochondrial protein COXIV (red) and PINK1 (green) in SMMC-7721 and HCCLM3 control and derived cells under the treatment of CCCP (10 μM) for 4 h. (Scale bars:10 μm) (C) mRNA levels of STOML2 and PINK1 were determined by qRT-PCR in SMMC-7721 and HCCLM3 with manipulated the expression of STOML2, taking GAPDH mRNA as a control. (D) The alteration of PINK1 protein in SMMC-7721 and HCCLM3 control and derived cells with co-treatment of 10μM CCCP and 20 μM MG132 was detected by Western blot. **P<0.01; ***P<0.001; ns, no significance.