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Additional file 5: Figure S5. of ROCK activity and the Gβγ complex mediate chemotactic migration of mouse bone marrow-derived stromal cells

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posted on 2015-07-24, 05:00 authored by Caroline Ryan, James Brown, Emer Bourke, Áine Prendergast, Claire Kavanagh, Zhonglin Liu, Peter Owens, Georgina Shaw, Walter Kolch, Timothy O’Brien, Frank Barry
CCR2, GPCR βγ subunit and PI3K activity is required for MCP-1-induced BMSC migration. a-c 3D migration of serum-starved BMSCs pre-treated with 10 μM RS102895, 10 μM MPS-Phos, 5 μM LY294002, or 10 μM Y27632 for 24 h prior to exposure to MCP-1. Representative migration traces shown of three separate independent experiments; 10 % fetal bovine serum included as a positive control. 3D three-dimensional, BMSC bone marrow-derived stromal cell, CCR chemokine (C motif) receptor, GPCR G protein-coupled receptor, MCP-1 monocyte chemoattractant protein 1, PI3K PI3 kinase. (PNG 273 kb)