13578_2021_707_MOESM2_ESM.tif (2.38 MB)
Additional file 2 of NME6 is a phosphotransfer-inactive, monomeric NME/NDPK family member and functions in complexes at the interface of mitochondrial inner membrane and matrix
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posted on 2021-11-18, 04:43 authored by Bastien Proust, Martina Radić, Nikolina Škrobot Vidaček, Cécile Cottet, Stéphane Attia, Frédéric Lamarche, Lucija Ačkar, Vlatka Godinić Mikulčić, Malgorzata Tokarska-Schlattner, Helena Ćetković, Uwe Schlattner, Maja Herak BosnarAdditional file 2: Figure. S1 Stable clones express a high amount of exogenous NME6-186-FLAG. a Ten micrograms of cell lysate from MDA-MB-231T (Ctrl) and stable clones overexpressing NME6-186-FLAG (KI-186-7, KI-186-18, KI-186-26) were analyzed by Western blot, using NME6 antibody. b Quantifications of endogenous NME6 (Ctrl) or exogenous NME6-186-FLAG (KIs) by densitometry were normalized to naphthol blue. Band intensities are displayed as a ratio of the hatched Ctrl sample. Note: The exogenous NME6-186-FLAG expression is ten to twenty time higher than the endogenous NME6 in Ctrl. Endogenous NME6 is undetectable in stable clones.
